GST anticorps (Rhodamine)

N° du produit ABIN1043858

N° du produit (Fournisseur): 600-400-200

L’anticorps Lapin Polyclonal anti-GST a été validé pour WB, FACS, FLISA et FM. Il convient pour détecter GST dans des échantillons de Schistosoma japonicum.

Aperçu rapide pour GST anticorps (Rhodamine) (ABIN1043858)

Antigène: GST (Glutathione S Transferase (GST))

Reactivité: Schistosoma japonicum

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GST est conjugé à/à la Rhodamine

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Détail du produit anti-GST anticorps (Rhodamine)

N° du produit (Fournisseur): 600-400-200

Fournisseur: Rockland

Fonction: GST Antibody Rhodamine Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Attributs du produit: Synonyms: rabbit anti-GST antibody Rhodamine conjugation, TRITC conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Purification: GST Antibody Rhodamine Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 2.4

Information d'application

Indications d'application:

Flow Cytometry Dilution: User Optimized

Application Note: GST Antibody Rhodamine Conjugated has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

FLISA Dilution: User Optimized

IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store GST Antibody Rhodamine Conjugated at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This GST Antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GST

Antigène: GST (Glutathione S Transferase (GST))

Autre désignation: GST

Sujet: Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.